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建立了将固相亲和层析与生物质谱技术相结合 ,分离提取鉴定给药猕猴血清中含有 6×His序列的重组人内皮抑制素的方法。用固相免疫亲和层析和金属螯合层析两种方法分别提取并富集血清中的重组药物 ,采用基质辅助激光解吸 电离飞行时间质谱的直接分析和肽质量指纹谱分析与数据库检索 ,分别对两种亲和提取方法得到的药物蛋白前体collagenXVIII[Homosapiens](IDofSWISS -PROT TrEMBL :Q8WXI5 )进行了鉴定
The method of combining solid phase affinity chromatography and bio-mass spectrometry was established, and the method of isolation and identification of recombinant human endostatin containing 6 × His sequence in macaque serum was established. Solid phase immunoaffinity chromatography and metal chelate chromatography were used to extract and enrich the recombinant drugs in serum separately. Matrix-assisted laser desorption / ionization time of flight mass spectrometry (MALDI-TOF MS) direct analysis and peptide mass fingerprinting (FTIR) The drug protein precursor collagenXVIII [Homosapiens] (IDofSWISS-PROT TrEMBL: Q8WXI5) obtained by the two affinity extraction methods was identified respectively