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首次在我国开展大规模的混配农药中毒流行病学调查,发现接触混配农药是当前发生农药中毒的重要危险因素,为针对性的预防提供了科学依据。毒理学研究证实多数农药混剂呈增毒效应或协同作用,且与其毒代动力学变化有密切关系,为今后开发高效、低毒的混配农药提供了毒理学依据。在国内外首次建立甲基对硫磷单克隆抗体酶联免疫测定法,并成功研制便携式血胆碱酯酶快速测定仪;这些检测接触、效应生物标志物的灵敏、特异、便捷的方法已试用于现场,并具有开发为产品的前景。同时还发现对氧磷酶基因在192位点的单核苷酸多态性,可能是甲基对硫磷免疫毒性作用的易感性生物标志物之一。在总结不同类别农药混配后所致中毒的临床表现特点和规律的基础上,修订现行的农药中毒诊断标准,增添了有机磷单剂及混剂中毒所致“中间期肌无力综合征”的新内容,将提高我国农药中毒的诊治水平。本研究主要成果达国内领先和国际先进水平。
For the first time in our country to carry out a large-scale epidemiological investigation of mixed pesticide poisoning and found that exposure to mixed pesticides is an important risk factor for pesticide poisoning, which provides a scientific basis for targeted prevention. Toxicology studies confirmed that most pesticide mixtures have an increased or synergistic effect and are closely related to their changes in toxicokinetics, providing a toxicological basis for the development of highly effective and low toxicity mixed pesticides in the future. The first time in home and abroad to establish methyl parathion-methyl monoclonal antibody enzyme-linked immunosorbent assay, and the successful development of portable blood cholinesterase rapid determination of these contacts; detection of biomarkers of effect, sensitive, specific and convenient method has been tested In the field, and has the prospect of developing for the product. At the same time, it was also found that the single nucleotide polymorphism of the paraoxonase gene at position 192 was probably one of the susceptible biomarkers of the immunotoxic effects of parathion methyl. On the basis of summarizing the characteristics and laws of the clinical manifestations of poisoning caused by different types of pesticides, the current diagnostic criteria of pesticide poisoning were revised and the “intermediate myasthenia syndrome” caused by organophosphate single agent and mixture poisoning was added New content, will improve the diagnosis and treatment of pesticide poisoning in China. The main achievements of this research reached the leading domestic and international advanced level.