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目的 研究不同盐的左氧氟沙星体外抗 HBV的作用及其机理。方法 以 HBs Ag、HBe Ag及细胞存活率为指标 ,评价药物体外抗 HBV效果 ;检测 HBV DNA、RNA的拷贝数 ,结合 Southern杂交 ,推测其体外抗HBV的作用机理。结果 磺酸左氧氟沙星对 HBs Ag和 HBe Ag的 ID5 0 分别为 63.1μg/m l及 81.3μg/ml,CD5 0 为182 .0 μg/ml,TI>2 ;对细胞内、外 HBV DNA及细胞内 HBV RNA均有剂量依赖性抑制作用 (P<0 .0 5 ) ;Southern杂交显示 ,其对细胞内 HBV DNA及复制中间体有抑制作用 ,对 sc DNA有部分抑制作用。结论 磺酸左氧氟沙星有抗 HBV作用 ,作用位点在 HBV DNA及 sc DNA水平
Objective To study the anti-HBV effect and mechanism of different salts of levofloxacin in vitro. Methods HBsAg, HBeAg and cell survival rate were used as indexes to evaluate the anti-HBV effect in vitro. The copy number of HBV DNA and RNA was detected, and the anti-HBV mechanism in vitro was estimated by Southern hybridization. Results The ID50 of levofloxacin sulfonate was 63.1μg / ml and 81.3μg / ml for HBsAg and HBeAg, respectively. The CD50 was 182.0μg / ml with TI> 2. The intracellular and extracellular HBV DNA and intracellular HBV RNA showed dose-dependent inhibition (P <0.05). Southern hybridization showed that it inhibited the intracellular HBV DNA and replication intermediates and partially inhibited the sc DNA. Conclusion Levofloxacin sulfonate has an anti-HBV effect and its site of action is at HBV DNA and sc DNA levels