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目的:研究鼻咽癌组织中DNA依赖蛋白激酶催化亚基(DNA-dependent protein kinase catalytic subunit,DNA-PKcs)和乳腺癌基因1(breast cancer 1,BRCA1)的蛋白表达及其临床意义。方法:收集2007-05-07-2011-11-23广西壮族自治区人民医院病理学确诊为鼻咽癌且应用调强放射治疗(intensity modulated radiation therapy,IMRT)的患者71例。采用免疫组化SP法检测71例患者中DNA-PKcs及BRCA1在鼻咽癌组织中的表达。阳性细胞计数≥50%分别定义为DNA-PKcs或BRCA1高表达组;阳性细胞计数<50%及不表达者分别定义为DNA-PKcs或BRCA1低表达组。并结合其临床资料,分析2种蛋白的表达与患者的急性放射反应、临床分期、肿瘤消退及生存时间的关系。结果:DNA-PKcs的表达与患者的AJCC分期(P=0.560)、皮肤(P=0.314)、黏膜(P=0.509)、涎腺急性放射损伤(P=0.403)及肿瘤消退情况(P=0.480)无显著相关性。BRCA1的表达与患者的AJCC分期(P=0.537)、皮肤(P=0.943)、黏膜(P=0.466)、涎腺急性放射损伤(P=0.651)及肿瘤消退情况(P=0.540)无显著相关性。DNA-PKcs表达与患者的生存时间呈正相关,r=0.234,P=0.048。结论:鼻咽癌组织中DNA-PKcs和BRCA1的表达与患者的临床分期、放射性损伤以及肿瘤的消退情况无关。DNA-PKcs低表达可能成为预测鼻咽癌患者预后较差的一个指标。
Objective: To investigate the protein expression of DNA-dependent protein kinase catalytic subunit (DNA-PKcs) and breast cancer 1 (BRCA1) in nasopharyngeal carcinoma and its clinical significance. Methods: Totally 71 patients were diagnosed as nasopharyngeal carcinoma with intensity modulated radiation therapy (IMRT) by People’s Hospital of Guangxi Zhuang Autonomous Region, 2007-05-07-2011-11-23. Immunohistochemical SP method was used to detect the expression of DNA-PKcs and BRCA1 in nasopharyngeal carcinoma in 71 patients. Positive cell counts ≥50% were defined as DNA-PKcs or BRCA1 high expression group; positive cell count <50% and no expression were defined as DNA-PKcs or BRCA1 low expression group. Combined with clinical data, the relationship between the expression of two proteins and acute radiation response, clinical stage, tumor regression and survival time were analyzed. Results: The expression of DNA-PKcs was significantly correlated with the AJCC stage (P = 0.560), skin (P = 0.314), mucosa (P = 0.509), acute salivary gland irradiation (P = ) No significant correlation. The expression of BRCA1 was not significantly associated with the stage of AJCC (P = 0.537), skin (P = 0.943), mucosa (P = 0.466), salivary gland acute radiation injury (P = 0.651) Sex. The expression of DNA-PKcs was positively correlated with the survival time of patients, r = 0.234, P = 0.048. Conclusion: The expression of DNA-PKcs and BRCA1 in nasopharyngeal carcinoma has nothing to do with the clinical stage, radiation injury and tumor regression. Low expression of DNA-PKcs may be a prognostic indicator of poor prognosis in patients with nasopharyngeal carcinoma.