目的 :测定复方环丙沙星注射液中环丙沙星和替硝唑的含量。方法 :采用反相 高效液相色谱法 ,色谱柱C18柱 ,流动相为 0 .4mol·L-1柠檬酸 甲醇 乙腈 (2∶1∶0 .8) ,流速为 0 .8ml·min-1,检测波长为 2 77nm。结果 :环丙沙星和替硝唑的回收率分别为 99.7%和 99.6 % ,RSD分别为 0 .9%和 0 .7%。结论 :方法快速、准确 ,可作为该复方制剂的质量控制标准 Objective: To determine the contents of ciprofloxacin and tinidazole in compound ciprofloxacin injection. Methods: Reversed-phase high performance liquid chromatography (HPLC) was used on a C18 column with a mobile phase of 0.4 mol·L-1 citric acid in methanol and acetonitrile at a flow rate of 0.8 ml · min- The detection wavelength is 2 77nm. Results: The recoveries of ciprofloxacin and tinidazole were 99.7% and 99.6%, respectively, with RSDs of 0.9% and 0.7%, respectively. Conclusion: The method is rapid and accurate and can be used as the quality control standard of this compound preparation