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本试验采用甲醇和蒸馏水浸提法,从69种青藏地区传统中草药中筛选具有诱导瓜列当(Phelipanche Aegyptiaca Pers.)和向日葵列当(Orobanche Cumana Wallr.)种子萌发作用的植物,旨在通过寻找稳定的种子萌发刺激物质来为根寄生杂草列当防除提供依据。结果表明:1)供试材料中,16种中草药的甲醇或蒸馏水浸提液诱导瓜列当种子的萌发率≥20%,21种中草药的甲醇或蒸馏水浸提液诱导向日葵列当种子的萌发率≥20%;2)其中,黄花蒿、雪参、鸡血藤、青兰、甘松和独一味6种中草药诱导2种列当的发芽率均高于20%,表明上述中草药种含有较高活性或较高浓度的列当种子萌发诱导物质,上述6种中草药可作为列当诱捕作物或列当种子萌发刺激物的潜在供体而应用于列当的生物防除。
In this study, methanol and distilled water extraction method was used to screen 69 Chinese traditional herbal medicines in Qinghai-Tibet region for plants that induce the germination of Phelipanche Aegyptiaca Pers. And Orobanche Cumana Wallr. Stable seed germination stimulating substances to provide a basis for the prevention and control of root parasitic weeds. The results showed that: 1) In the tested materials, the methanol or distilled water extracts of 16 kinds of Chinese herbs induced the germination rate of melon seeds to be ≥20%, and the methanol and distilled water extracts from 21 kinds of Chinese herbs induced the germination rate ≥20%; 2) Wherein, the germination rate of two kinds of Chinese Herbal Medicine induced by 6 kinds of herbs of Artemisia annua L., Radix Ginseng, Millettia, Qinglan, Nansong and Yiduyiwere higher than 20%, indicating that the above Chinese herbal medicines contain higher Active or higher concentration of seed germination inducing substances, the above six kinds of Chinese herbal medicine can be used as a trap when the trapping crop or seed as a potential stimulus for the donor potential applied biocontrol when the column.