论文部分内容阅读
１目的建立从孕妇外周血中分离胎儿细胞进行遗传病非损伤性产前基因诊断的新策略。２方法孕妇外周血，经三密度梯度离心分离，有核红细胞用联结抗ＣＤ７１单抗的微磁珠进行磁性激活的细胞分离（ＭＡＣＳ）方法富集ＣＤ７１＋细胞，经免疫组化染色，用显微操作仪控制的玻璃针挑
OBJECTIVE: To establish a new strategy of non-invasive prenatal genetic diagnosis of genetic diseases by isolating fetal cells from peripheral blood of pregnant women. 2 method of peripheral blood of pregnant women, separated by three density gradient centrifugation, nucleated erythrocytes were enriched in CD71 + cells by magnetic activated cell separation (MACS) method using micro-magnetic beads linked to anti-CD71 monoclonal antibody, immunohistochemical staining, Operators control the glass needle pick