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[目的]探讨改善酒精性胰腺腺泡细胞分泌功能不足的分子靶点。[方法]1将48只大鼠随机分为4组,每组12只。饮酒6个月组(A1组),饮酒6个月对照组(A2组),戒酒3个月组(B1)组,戒酒3个月对照组(B2组);2苏木精-伊红染色和电镜观察戒酒后酒精性胰腺腺泡细胞的形态变化;3采用酶法测定胰腺组织的淀粉酶、脂肪酶含量;4免疫荧光法和免疫印迹检测CCK-A受体在胰腺的表达;5放射免疫分析法(RIA)测定胰腺和小肠组织中的胆囊收缩素(CCK)含量;6用比色法测定血浆和小肠食糜中游离脂肪酸及食糜中三酰甘油含量。[结果]与A2组大鼠相比,A1组电镜下见腺泡细胞超微结构出现了明显的变化,如线粒体水肿,髓样小体形成,粗面内质网扩张,酶原颗粒减少,易见脂滴;A1组大鼠饮酒6个月后胰腺淀粉酶、脂肪酶水平、小肠和胰腺的CCK水平均较A2组显著下降。B1组胰腺组织学及腺泡细胞超微结构的病变较B2组并无明显改善,其胰腺淀粉酶、脂肪酶水平、小肠和胰腺的CCK水平仍低于B2组。[结论]慢性酒精对胰腺腺泡细胞结构及功能的损伤在戒酒后仍不能自然恢复;补充消化酶,可改善其外分泌功能。
[Objective] To explore the molecular targets for improving the secretion of alcohol-induced pancreatic acinar cells. [Method] 1 48 rats were randomly divided into 4 groups of 12 rats. (Group A1), alcohol consumption for 6 months (group A2), alcohol withdrawal for 3 months (group B1) and alcohol withdrawal for 3 months (group B2); 2 Hemujing-Yi The morphological changes of alcohol-induced pancreatic acinar cells were observed by red staining and electron microscopy.3 The content of amylase and lipase in pancreas was measured by enzyme-linked immunosorbent assay.4 The expression of CCK-A receptor in pancreas was detected by immunofluorescence and immunoblotting ; 5 Radioimmunoassay (RIA) was used to determine the content of cholecystokinin (CCK) in pancreas and small intestine; 6 The content of triglycerides in free fatty acids and chyme in plasma and intestinal chyme was determined by colorimetry. [Results] The ultrastructure of acinar cells showed obvious changes under the electron microscope in A1 group compared with A2 group, such as mitochondria edema, medullary body formation, rough endoplasmic reticulum dilation, reduction of zymogen granules, Easy to see lipid droplets; A1 group 6 months after drinking alcohol, pancreatic amylase, lipase levels, small intestine and pancreas CCK levels were significantly decreased compared with the A2 group. The histopathology of pancreas and the ultrastructure of acinar cells in B1 group were not significantly improved compared with those in B2 group. The levels of amylase, lipase, CCK in small intestine and pancreas in group B1 were still lower than those in group B2. [Conclusion] Chronic alcohol can not restore the structure and function of pancreatic acinar cells spontaneously after abstinence from alcohol consumption. Supplementation of digestive enzymes can improve the exocrine function.