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目的:观察扶正利湿活血中药复方对鸭乙型肝炎病毒(DHBV)的抑制作用。方法:PCR筛选先天感染DHBV的1日龄广东麻鸭,随机分为病毒对照组、拉米夫定组、扶正利湿活血复方高、低剂量组,每组7只,各组分别灌胃给药30天。在灌胃前、灌胃第10、20、30天及停药第5天分别采血,辛酸钠法提取血清DHBV DNA,采用荧光定量PCR法检测血清DHBV DNA的含量。结果:扶正利湿活血复方高剂量组在给药后的第10、20、30天时,鸭血清病毒载量均明显下降,(与D0比较,P<0.05;各时间点与病毒对照组比较,P<0.01);在停药5天后,与D0组内比较,血清病毒载量仍然显著降低(P<0.01),但与病毒对照组比较则无显著下降(P>0.05);扶正利湿活血复方低剂量组在给药后的第10、20、30天时,鸭血清病毒载量均明显下降,(与D0比较,P<0.01;各时间点与病毒对照组比较,P<0.01);在停药5天后,组内与D0比较及组间与病毒对照组比较均明显下降(P<0.05)。结论:扶正利湿活血复方可抑制DHBV DNA的复制,低剂量长期效应更为明显。
Objective: To observe the inhibitory effect of Fuzheng Lishi Huoxue Compound on Duck Hepatitis B virus (DHBV). Methods: One-day-old Guangdong duck with DHBV congenital infection was screened and randomly divided into high-dose group and low-dose group of virus control group, lamivudine group and Fuzheng Lishi Huoxue Compound group, with 7 rats in each group. Medicine for 30 days. Serum DHBV DNA was extracted by sodium octanoate before gavage, on the 10th, 20th and 30th days of gavage, and on the fifth day of withdrawal. Serum DHBV DNA content was detected by fluorescence quantitative PCR. Results: The duck serum viral load decreased significantly on the 10th, 20th and 30th day after administration (P <0.05 compared with D0). Compared with the virus control group, (P <0.01). After 5 days of withdrawal, the serum viral load was still significantly lower than that in D0 group (P <0.01), but no significant decrease compared with virus control group (P> 0.05) In the compound low-dose group, duck serum viral load decreased significantly at the 10th, 20th and 30th day after administration (P <0.01 compared with D0; P <0.01 at each time point compared with the virus control group) After stopping for 5 days, the intra-arteries and the D0 were significantly lower than those in the control group (P <0.05). Conclusion: Fuzheng Lishi Huoxue Compound can inhibit the replication of DHBV DNA, and the long-term effect of low dose is more obvious.