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经短期培养孵化的人体羊膜、绒毛膜、蜕膜和胎盘弥散细胞合成和代谢了前列腺素(PG_s)E、F和6-酮PGF_(1α)。调节PG_s合成和代谢的激素是雌激素。近来已证明17-β雌二醇(E_2)及其代谢产物2-羟17-β雌二醇(2-羟E_2)同时刺激PGE和PGF的合成而抑制6-酮PGF_(1α)产生。研究对象为7例足月作选择性剖腹产和4例自然临产阴道分娩孕妇。婴儿娩出后,人工剥离羊膜与绒毛膜。用刀片或玻片从绒毛膜刮下蜕膜,以生理盐水冲洗几次。然后将组织放入37℃的振荡水浴,在空气中孵育20～30分钟。除去组织和悬浮液,将沉淀物在新鲜的浴液中再孵育1至1.5小时。滤出分散的细胞,用不含Ca~(2+),Mg~(2+)的磷酸盐溶液(MEM)离心洗两次(最终可存活的细胞浓度
Prostaglandin (PG_s) E, F and 6-keto PGF_ (1α) were synthesized and metabolized by short-term incubation of human amniotic membrane, chorion, decidua and placental cells. The hormone that regulates PG synthesis and metabolism is estrogen. It has recently been shown that 17-β estradiol (E 2) and its metabolite 2-hydroxy 17-β estradiol (2-hydroxy E 2) simultaneously stimulate PGE and PGF synthesis and inhibit 6-keto PGF_ (1α) production. The subjects were 7 full-term cesarean section and 4 spontaneous vaginal delivery pregnant women. After the baby is delivered, artificial amniotic membrane and chorion are stripped. The decidua is scraped off the chorion with a razor blade or slide and rinsed several times with saline. The tissue is then placed in a shaking water bath at 37 ° C and incubated in air for 20-30 minutes. The tissue and suspension are removed and the pellet is incubated for a further 1 to 1.5 hours in fresh bath. The dispersed cells were filtered off and washed twice with a phosphate buffer solution (MEM) containing neither Ca2 + nor Mg2 + (the final viable cell concentration