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目的探索小剂量环磷酰胺长期慢性腹腔注射建立大鼠间质性膀胱炎(interstitial cystitis,IC)模型的方法,并研究阿米替林对IC的治疗作用。方法 42只SD大鼠,采用随机数字表法分为7组:对照组,4、48 h模型组,10、30、45 d模型组和治疗组。模型组和治疗组腹腔注射环磷酰胺(cyclophosphamide,CYP)40 mg/kg,治疗组于第31天开始给予阿米替林10 mg/(kg·d)灌胃,持续15 d。观察膀胱组织大体、光镜及电镜形态学表现。通过ELISA检测对照组和30、45 d模型组以及治疗组血清中IL-6、IL-10、IL-17A水平的变化。结果模型组大鼠表现出IC的病理学变化。30、45 d模型组血清中IL-6、IL-10、IL-17A水平均较对照组升高(P<0.05);治疗组较45 d模型组降低,但高于对照组,差异均有统计学意义(P<0.05)。结论环磷酰胺小剂量慢性作用可构建大鼠IC模型,阿米替林可以改善IC大鼠的病理学表现。
Objective To explore a long-term chronic intraperitoneal injection of interstitial cystitis (IC) model of low dose cyclophosphamide and to study the therapeutic effect of amitriptyline on IC. Methods 42 SD rats were randomly divided into 7 groups: control group, 4,48 h model group, 10, 30, and 45 d model group and treatment group. The model group and the treatment group were intraperitoneally injected with cyclophosphamide (CYP) 40 mg / kg, and the treatment group was started on the 31st day with amitriptyline 10 mg / (kg · d) orally for 15 days. Observe the bladder tissue, light microscopy and electron microscopy morphological findings. The levels of IL-6, IL-10 and IL-17A in sera of control group, 30,45 d model group and treatment group were detected by ELISA. Results The rats in model group showed the pathological changes of IC. The serum levels of IL-6, IL-10 and IL-17A in model group at 30 and 45 d were significantly higher than those in control group (P <0.05). The treatment group was lower than the model group at 45 d, but higher than the control group Statistical significance (P <0.05). Conclusion A small dose of cyclophosphamide can establish a rat model of chronic IC. Amitriptyline can improve the pathological manifestations of IC rats.