台盼兰拒染法测定肿瘤细胞对化疗药物的敏感和耐受情况,方法简单而快速,但细胞活力结果偏高。本文将染料改为固绿,同时加鸭红细胞作为内标,以矫正细胞崩解和增殖导致的细胞活力偏高,从而对台盼兰拒染法进行改进。 The trypan blue dye exclusion method was used to determine the sensitivity and tolerance of tumor cells to chemotherapeutic drugs. The method was simple and rapid, but the cell viability results were high. In this article, the dye was changed to Fast Green, and red blood cells were added as internal standards to correct cell disintegration and proliferation resulting in high cell viability, thus improving trypan blue exclusion.