论文部分内容阅读
蛋白质因其多样性和功能性,是生物体内一类非常重要的分子.通常蛋白质的表征需要借助荧光或者酶的标记.而纳米孔技术,得益于免标记、单分子检测等优势,为蛋白质的表征提供了新方向.我们使用固态纳米孔完成了单个蛋白质分子及蛋白质-蛋白质结合物的检测.可以发现,外部电压和电解质溶液的酸碱度会直接影响蛋白质分子表面带电量,从而加快或延迟其在孔内的易位时间.抗原、抗体本质上都是蛋白质,两者之间具有高度特异性.通过比较抗体溶液在添加特异性抗原前后的易位事件,实现了单个蛋白质分子和蛋白质-蛋白质结合物的区分.未来,纳米孔技术有望应用于多蛋白质分子的辨识、蛋白质分子相互作用机制等方面的研究.
Proteins are a very important class of molecules in organisms due to their diversity and functionality. Usually protein characterization requires fluorescence or enzyme labeling, whereas nanopore technology benefits from the advantages of label-free and single-molecule detection for proteins Characterization of the protein provides a new direction.We used solid-state nanopore to complete a single protein and protein protein conjugate detection.It can be found that the external voltage and electrolyte solution pH will directly affect the surface charge of protein molecules to accelerate or delay its The translocation time in the wells, the antigen and the antibody are all proteins in nature, with a high degree of specificity between the two. By comparing the antibody solution translocation events before and after the addition of specific antigens, a single protein molecule and protein- In the future, nanopore technology is expected to be applied to the identification of multi-protein molecules and the interaction between protein molecules and other aspects.