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目的:研究慢性应激对正常及糖尿病倾向小鼠各组织抗氧化酶影响的差异并探讨其意义。方法:用链脲佐菌素(STZ)复制糖尿病倾向小鼠模型(STZ鼠),予以6周慢性多相性应激(限制、旋转、拥挤),检测其空腹血糖(FBG)、空腹胰岛素(FINS)水平和胰、心、肝组织超氧化物歧化酶(SOD)、谷肽甘肽过氧化物酶(GSH-PX)活性及脂质过氧化物(LPO)含量。结果:慢性应激使STZ鼠FBG水平升高,FINS水平未增高;对STZ鼠各组织抗氧化酶活性有不同影响:加重其胰腺SOD活性下降和LPO含量升高,并使其GSH-PX活性降低(P均<0.05),对其肝脏显示使SOD、GSH-PX活性升高和LPO含量下降的相反作用(P均<0.05)。结论:慢性应激及STZ诱导的糖尿病倾向协同作用特异地加重小鼠胰腺抗氧化酶活性下降和脂质过氧化物形成,可能参与损害其胰岛素分泌功能和导致高血糖症。
OBJECTIVE: To study the differences of antioxidant enzymes in different tissues of normal and diabetic mice induced by chronic stress and to explore its significance. Methods: STZ mice were induced by streptozotocin (STZ) and were subjected to chronic multiphasic stress (restriction, rotation and crowding) for 6 weeks. The levels of fasting blood glucose (FBG), fasting insulin ), And the activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-PX) and lipid peroxides (LPO) in pancreas, heart and liver were measured. Results: Chronic stress increased the levels of FBG and the level of FINS in STZ mice, but had different effects on the activities of antioxidant enzymes in STZ mice: increased activity of SOD and activity of GSH-PX (P <0.05). The liver showed the opposite effect of increasing SOD, GSH-PX activity and decreasing LPO content (all P <0.05). CONCLUSION: Chronic stress and systolic diabetes mellitus induced by STZ specifically increase the activity of antioxidant enzymes and the formation of lipid peroxides in the pancreas of mice, which may be involved in impairing insulin secretion and causing hyperglycemia.