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目的:研究EBV膜蛋白gp350/220的表达对共刺激分子ICOS的影响以及与T细胞淋巴瘤的关系。方法:繁殖饲养BLLF-1转基因昆明鼠以及正常昆明鼠,观察它们淋巴瘤发病率的差异。取发病的BLLF-1转基因昆明鼠脾脏淋巴细胞,用FITC标记的抗gp350/220单克隆抗体进行免疫荧光染色,检测gp350/220是否在该转基因昆明鼠淋巴细胞内表达及其表达部位。对发病转基因昆明鼠组织进行免疫组化染色,并与正常昆明鼠的进行对比分析。用RT-PCR方法检测转基因小鼠共刺激分子ICOS的表达变化。结果:BLLF-1转基因昆明鼠淋巴组织病理性改变与正常昆明鼠有显著差异,免疫荧光检测到该转基因小鼠淋巴细胞表达gp350/220于胞浆和胞膜上,病理学观察发现,发病小鼠淋巴结组织有反应性增生,脾脏淋巴瘤细胞浸润,免疫组化证明为T细胞淋巴瘤,转基因小鼠脾脏、肺脏及肿瘤中ICOS表达显著升高。结论:BLLF-1基因的表达,与该转基因小鼠发生T细胞淋巴瘤有关,并引起共刺激分子ICOS表达的变化,该转基因小鼠的建立,为我们进一步研究BLLF-1基因在T细胞淋巴瘤发病中的作用提供了良好的动物模型。
Objective: To investigate the effect of EBV membrane protein gp350 / 220 on costimulatory molecule ICOS and its relationship with T-cell lymphoma. Methods: Breeding BLLF-1 transgenic Kunming mice and normal Kunming mice to observe the difference of their incidence of lymphoma. The spleen lymphocytes from infected BLLF-1 transgenic Kunming mice were harvested and immunofluorescent staining was performed with FITC-labeled anti-gp350 / 220 monoclonal antibody to detect whether gp350 / 220 was expressed in lymphocytes of the transgenic Kunming mouse and its expression site. Immunohistochemical staining of the Kunming mice infected with the gene was performed and compared with that of the normal Kunming mice. The expression of costimulatory molecule ICOS in transgenic mice was detected by RT-PCR. Results: The pathological changes of lymphoid tissues in BLLF-1 transgenic Kunming mice were significantly different from those in normal Kunming mice. Immunofluorescence showed that gp350 / 220 was expressed in the cytoplasm and plasma membrane of lymphocytes in the transgenic mice. Pathological observation showed that the incidence of small Rat lymph node tissue reactive hyperplasia, spleen lymphoma cell infiltration, immunohistochemistry proved T-cell lymphoma, transgenic mice spleen, lung and tumor ICOS expression was significantly increased. CONCLUSIONS: The BLLF-1 gene expression is associated with T-cell lymphoma in this transgenic mouse and causes changes in ICOS expression of co-stimulatory molecules. The establishment of this transgenic mouse is a useful tool for further study of BLLF- The role of neoplasia provides a good animal model.