论文部分内容阅读
探讨动脉粥样硬化性脑梗死（ACI）与载脂蛋白B基因XbaⅠ酶切多态性的关系。方法：以apoBcDNA片段（LB1．5）为探针，利用Southern印迹杂交技术，检测了56名汉族ACI患者和61名正常对照者的apoB基因XbaⅠ限制性片段长度多态性（RFLP）等位基因频率。结果：发现8．6kb（X~-）和5．0kb（X~+）两条杂交片段，X~+等位基因频率在ACI（0．143）和对照者（0.066）之间差异有显著性（x~2=4．28，P＜0．05）。结论：5．0kb片段可能与ACI有关。
To investigate the relationship between atherosclerotic cerebral infarction (ACI) and Xba Ⅰ digestion polymorphism of apolipoprotein B gene. Methods: Southern blotting was used to detect the allele of Xba Ⅰ restriction fragment length polymorphism (RFLP) of apoB gene in 56 Han Chinese ACI patients and 61 healthy controls using apoB cDNA fragment (LB1.5) as a probe. frequency. Results: The frequencies of X ~ + alleles were found to be significantly different between ACI (0.143) and control (0.066), which were found in the two fragments of 8.6kb (X ~ -) and 5.0kb (X ~ 2 = 4.28, P <0.05). Conclusion: 5.0kb fragment may be related to ACI.