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目的探讨 K-Cl 协同转运子1(KCC1)在宫颈癌组织中的表达及在宫颈癌发生、发展中的作用。方法采用半定量逆转录-聚合酶链反应(RT-PCR)、Western 印迹及免疫荧光染色方法测定40例宫颈癌、10例宫颈上皮内瘤样病变(CIN)和10例慢性宫颈炎组织中 KCC1mRNA 及蛋白的表达及定位情况。结果宫颈癌组织中 KCC1mRNA 和蛋白的表达(灰度比值分别为1.29±0.17,1.18±0.27)均明显高于慢性宫颈炎(0.59±0.27,0.33±0.18)、CIN 组织(0.50±0.28,0.27±0.15),差异有统计学意义(均 P<0.05)。宫颈低、中分化癌组织中 KCC1mRNA 及蛋白的表达(灰度比值分别为1.39±0.12,1.37±0.15)明显高于高分化组(1.16±0.14,0.96±0.19),差异有统计学意义(P<0.05);KCC1mRNA 及蛋白在晚期宫颈癌组织中的表达与早期组差异无统计学意义,KCC1蛋白在宫颈癌及对照组织中的分布定位在细胞膜上。结论 KCC1基因可能在宫颈癌的发生发展中起重要的作用。
Objective To investigate the expression of K-Cl co-transporter 1 (KCC1) in cervical cancer and its role in the development and progression of cervical cancer. Methods The expressions of KCC1 mRNA in 40 cases of cervical cancer, 10 cases of cervical intraepithelial neoplasia (CIN) and 10 cases of chronic cervicitis were detected by semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR), Western blot and immunofluorescence staining And protein expression and location. Results The expressions of KCC1 mRNA and protein in cervical cancer tissues were significantly higher than those of chronic cervicitis (0.59 ± 0.27,0.33 ± 0.18, 0.50 ± 0.28, 0.27 ± 0.18, respectively) 0.15), the difference was statistically significant (all P <0.05). The expression of KCC1mRNA and protein in low and moderately differentiated cervical cancer tissues was significantly higher than that in well-differentiated group (1.16 ± 0.14,0.96 ± 0.19, P <0.01), the difference was statistically significant (P <0.05). The expression of KCC1 mRNA and protein in advanced cervical cancer tissues was not significantly different from that in early stage. The distribution of KCC1 protein in cervical cancer and control tissues was located on the cell membrane. Conclusion KCC1 gene may play an important role in the development of cervical cancer.