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目的:建立同时测定北沙参中补骨脂素、花椒毒素、异茴芹内酯、佛手柑内酯和东莨菪内酯5种香豆素类成分的胶束电动毛细管电泳法。方法:采用未涂层融硅石英毛细管柱(50.2 cm×75μm×40 cm),20 mmol.L-1硼砂溶液-16 mmol.L-1十二烷基硫酸钠(SDS)-15%乙腈(pH9.6)为运行缓冲液,分离电压22 kV,紫外检测波长214 nm。对缓冲液种类、浓度、pH、SDS、乙腈浓度、电压、温度等因素对分离的影响做了系统的考察。结果:补骨脂素、花椒毒素、异茴芹内酯、佛手柑内酯和东莨菪内酯在7 min内全部分离,线性范围分别为9.91～82.6,37.2～162,2.23～18.6,2.73～22.3,2.89～20.1 mg.L-1,平均回收率分别为98.9%,98.4%,101.3%,99.1%,98.0%。结论:该方法为北沙参药材的质量评价提供了新的途径,简便、快速、灵敏、准确。
OBJECTIVE: To establish a micellar electrokinetic capillary electrophoresis (CE-Capillary electrophoresis) method for the simultaneous determination of five coumarins in Pseudostellariae Setariae, including psoralen, xanthotoxin, anison lactone, bergamot and scopoletin. Methods: Uncoated fused silica capillary column (50.2 cm × 75 μm × 40 cm), 20 mmol·L-1 borax solution-16 mmol·L-1 sodium dodecyl sulfate (SDS) pH9.6) for the running buffer, the separation voltage of 22 kV, UV detection wavelength of 214 nm. The effects of buffer type, concentration, pH, SDS, acetonitrile concentration, voltage and temperature on the separation were systematically investigated. Results: The psoralen, fenvalin, anisan lactone, bergapten and scopoletin were all separated within 7 min. The linear range was 9.91 ~ 82.6, 37.2 ~ 162, 2.23 ~ 18.6 and 2.73 ~ 22.3,2.89 ~ 20.1 mg.L-1, the average recoveries were 98.9%, 98.4%, 101.3%, 99.1%, 98.0%, respectively. Conclusion: This method provides a new way for the quality evaluation of Radix isatidis, which is simple, rapid, sensitive and accurate.