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通过开顶式气室研究了CO2浓度倍增(750vs.375μL/L)对取食转Bt水稻及其对照亲本秀水11的二化螟Chilo suppressalis(Walker)幼虫体内代谢酶乙酰胆碱酯酶(AChE)和保护酶(超氧化物歧化酶SOD、过氧化物酶POD和过氧化氢酶CAT)活力的影响,以期明确该类害虫对大气CO2浓度升高及转Bt水稻的生理响应。研究结果表明:(1)AChE受CO2浓度水平影响显著,与对照CO2处理相比,高CO2条件下该酶活力显著降低;(2)高CO2水平及水稻中Bt毒素蛋白的存在均会导致二化螟幼虫体内POD活力显著降低,SOD初期活力显著升高,但不能维持较长时间,CAT活力表现为先升后降的特点;(3)当受到水稻品种、CO2浓度水平和取食时间的综合影响时,以上代谢酶和保护酶活力会发生显著变化。短时间内二化螟幼虫可通过体内以上生理代谢调节减缓受CO2浓度升高和水稻中Bt毒素蛋白的不利影响,但长时间环境胁迫下这种应对不利环境的响应机制将会失去作用,最终导致种群适合度的降低。
The effects of doubled CO2 concentration (750 vs.375 μL / L) on the activity of acetylcholinesterase (AChE) and the activity of acetylcholinesterase (AChE) in Chilo suppressalis (Walker) larvae fed on transgenic Bt rice and its parental parent Xiashui 11 (Superoxide dismutase SOD, peroxidase POD and catalase CAT) in order to clarify the effects of these pests on the atmospheric CO2 concentration and the physiological response of transgenic Bt rice. The results showed that: (1) AChE was significantly affected by CO2 concentration, and the activity of AChE was significantly decreased under high CO2 compared with that of control CO2 treatment; (2) High CO2 level and the presence of Bt toxin protein in rice all resulted in two The activities of POD in the larvae of the rice borer were significantly decreased, the initial activities of SOD increased significantly, but they could not maintain for a long time and CAT activity increased first and then decreased. (3) When the rice varieties, CO2 concentration and feeding time When combined, the above metabolic enzyme and protective enzyme activity will change significantly. In short time, the stem borer larvae can slow down the increase of CO2 concentration and the negative effect of Bt toxin protein in rice through the above physiological and metabolic regulation, but this response mechanism to adverse environment will lose their effect under long-term environmental stress, Resulting in reduced population fitness.