论文部分内容阅读
目的建立大剂量盐酸异丙肾上腺素(Iso)诱发大鼠心肌缺血性坏死模型。方法多点皮下注射Iso15mg/kg,建立大鼠心肌缺血坏死模型,并检测其心电图(ECG)、血清酶学指标、心肌血清羟脯氨酸含量、三磷酸腺苷(ATP)含量及HE染色结果。结果与正常对照组比较,模型组大鼠心电图各点ST段和T波均有明显的变化;注射Iso后4h,乳酸脱氢酶(LDH)、肌酸激酶(CK)、肌酸激酶同工酶(CK-MB)水平达高峰;注射后6h,天门冬氨酸氨基转移酶(AST)水平达高峰;随着注射后时间的延长和病变程度的加重,心肌血清羟脯氨酸含量明显增加;ATP含量明显降低;注射Iso2h后,模型组即出现小的坏死灶,且随时间的延长,病变程度明显加重,坏死面积在3周达到最大,并出现明显的纤维化。结论一次性皮下多点注射15mg/kg的Iso,可成功地诱发大鼠心肌缺血性坏死,且可出现明显的纤维化。
Objective To establish a rat model of myocardial ischemic necrosis induced by high dose of isoprenaline hydrochloride (Iso). Methods Iso15mg / kg was injected subcutaneously to establish model of myocardial ischemia and necrosis in rats. The electrocardiogram (ECG), serum enzyme, serum hydroxyproline, ATP content and HE staining were determined. Results Compared with the normal control group, ST segment and T wave in each point of electrocardiogram in model group had obvious changes. After 4h injection of Iso, lactate dehydrogenase (LDH), creatine kinase (CK), creatine kinase (CK-MB) reached its peak. The level of aspartate aminotransferase (AST) peaked at 6h after injection. The content of hydroxyproline in myocardium increased significantly with the prolongation of time and the degree of lesion after injection ; The content of ATP was significantly reduced; after injection of Iso2h, the model group showed a small necrosis, and with the extension of time, the severity of the disease increased significantly, the area of necrosis reached the maximum in 3 weeks, and significant fibrosis. Conclusion Single injection of 15mg / kg Iso subcutaneously successfully induced myocardial ischemic necrosis in rats, and obvious fibrosis could occur.