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在同工酶研究基础上,利用随机扩增多态性DNA(RAPD)及DNA扩增指纹(DAF)方法,从生态学观点分析了北京东灵山区辽东栎(QuercusliaotungensisKoidz.)种群的遗传结构和多样性。对12个引物扩增产生的205个产物(部分),经Shannon信息指数分析,结果表明:辽东栎种群内部存在丰富的遗传变异,中央种群的遗传多样性高于边缘种群的遗传多样性。总遗传多样性的95%发生在种群内,只有5%发生在种群间。两种群在个别位点上存在差异。不同年龄组辽东栎的分析结果表明:人为干扰对辽东栎的遗传结构有一定影响。
Based on the study of isozymes, the genetic structure of Quercusliaotungensis Koidz. Populations from Dongling Mountain area in Beijing was analyzed by using random amplified polymorphic DNA (RAPD) and DNA fingerprinting (DAF) Diversity. The results of Shannon’s information index analysis of 205 products (partial) amplified by 12 primers showed that there were abundant genetic variation in Q. liaotungensis population, and the genetic diversity of central population was higher than that of marginal population. 95% of total genetic diversity occurs within the population and only 5% occurs among the populations. There are differences between the two groups at individual sites. The results of the analysis of Quercus liaotungensis in different age groups showed that human disturbance had a certain influence on the genetic structure of Quercus liaotungensis.