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为了保持优良无性系的遗传特性,研究腋芽直接形成芽的快速增殖方法,对基本培养基N、K等营养元素进行优选试验,筛选出最佳的初次培养基:改良的MS(1/4NH_4NO_3,1/4KNO_3,其他成分不变)+BA0.2mg/L+NAA0.01mg/L+蔗糖2.5%;继代培养基:改良的MS(1/4NH_4NO_3,1/4KNO_3,增加KH_2PO_450mg/L,其它成分不变)+BA0.2mg/L+蔗糖2.5%和生根培养基:改良的MS(1/4NH_4NO_3,其他成分不变)+NAA0.03mg/L+蔗糖1.5%。组培苗芽苗不发生玻璃化,生长粗壮,1个芽一年可增殖近2万株;生根率98%以上,根系良好;全光喷雾移栽,成活率达83.06%。
In order to maintain the genetic characteristics of elite clones, the rapid proliferation of axillary buds directly bud formation was studied. The optimal nutrient mediums such as N, K were selected and the best primary culture mediums were screened: modified MS (1 / 4NH_4NO_3, 1 / 4KNO_3, the other ingredients remain unchanged) + BA0.2mg / L + NAA0.01mg / L + sucrose 2.5%; subculture medium: modified MS (1 / 4NH_4NO_3, 1 / 4KNO_3, increase KH_2PO_450mg / L, BA0.2mg / L + sucrose 2.5% and rooting medium: modified MS (1 / 4NH4NO3, other components unchanged) + NAA0.03mg / L + sucrose 1.5%. The bud of tissue culture seedlings did not vitrify, grow thick, a bud can proliferate nearly 20000 a year; rooting rate of 98%, root system is good; all-optical spray transplanting survival rate of 83.06%.