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目的:探讨布鲁氏菌病患者体内可溶性T细胞免疫球蛋白黏蛋白分子3(s Tim-3)、高迁移率族蛋白1(HMGB1)和转化生长因子-β(TGF-β)的变化特点,分析这些变化与布鲁氏菌感染的关联性。方法:28例未经治疗布鲁氏菌病患者为布病病例组,28例在年龄和性别上与布病病例组匹配的健康人为对照组,采用ELISA法检测了布病病例组和对照组血清中s Tim-3和HMGB1的水平,通过ELISPOT法检测了这些患者外周血单个核细胞(PBMC)经布鲁氏菌抗原刺激产生TGF-β的斑点形成细胞(SFC)。结果:与正常对照组相比,布鲁氏菌病患者血清中s Tim-3和HMGB1水平及PBMC中产生TGF-β的SFC均明显增高(P<0.01);产生TGF-β的SFC与血清HMGB1水平呈正相关性(P<0.05)。结论:布鲁氏菌病患者血清中s Tim-3和HMGB1升高,且在布鲁氏菌抗原刺激后PBMC中产生TGF-β的SFC亦升高,这些分子可能参与了布鲁氏菌感染发生发展的过程,并与免疫逃避有关。
Objective: To investigate the changes of s Tim-3, HMGB1 and TGF-β in patients with brucellosis , Analyze the association of these changes with brucellosis. Methods: 28 cases of untreated brucellosis patients were brucellosis cases, 28 cases of age and sex matched brucellosis cases were healthy control group. ELISA method was used to test the cases of brucellosis and control group Serum levels of s Tim-3 and HMGB1 were measured by ELISPOT in peripheral blood mononuclear cells (PBMCs) of these patients stimulated with Brucella antigens to produce TGF-β-speckle-forming cells (SFCs). Results: Compared with the normal control group, the levels of s Tim-3 and HMGB1 in sera of patients with brucellosis and the levels of TGF-β in PBMC were significantly increased (P <0.01); SFC and serum HMGB1 level was positively correlated (P <0.05). CONCLUSIONS: The levels of s Tim-3 and HMGB1 in serum of patients with brucellosis are elevated, and the SFC of TGF-β produced by PBMCs stimulated by Brucella antigens is also elevated. These molecules may be involved in brucellosis The development of the process, and immune evasion.