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在分离鉴定毛竹(Phyllostachys edulis)根部相关内生菌的过程中,筛选得到具有生物活性的菌株SHEN20121。通过对其16S rDNA序列的分析,结果显示其与类芽孢杆菌属中97个细菌的序列相似性为97%~99%;系统学分析显示该菌株与多粘类芽孢杆菌聚在一个分支上,bp支持率为100,因此菌株SHEN20121被鉴定为多粘类芽孢杆菌(Paenibacillus polymyxa)。通过平板对峙实验证明了菌株SHEN20121对多种植物病原真菌:尖孢镰孢(Fusaricum oxysporum),互隔链格孢(Alternaria alternata),立枯丝核菌(Rhizoctonia solani),灰葡萄孢(Botrvtis cinerea),小孢拟盘多毛孢(Pestalotiopsis microspora),柑橘青霉病菌(Penicillium italicum)均具有抑制作用,其中对灰葡萄孢,小孢拟盘多毛孢和柑橘青霉病菌的抑菌效果明显,尤其是首次发现对拟盘多毛孢属病原菌的抑制作用。作者认为该菌在生物农药方面具有潜在的应用价值,并可能将在植物病害的生物防治方面发挥重要作用。
During the isolation and identification of endophytes related to the roots of Phyllostachys edulis, the biologically active strain SHEN20121 was screened out. The 16S rDNA sequence analysis revealed 97% -99% similarity to 97 bacteria in the genus Paenibacillus. Phylogenetic analysis showed that the strain and the B. polymyxa clustered in one branch, The bp support was 100, so strain SHEN20121 was identified as Paenibacillus polymyxa. The plate stand-off test demonstrated that the strain SHEN20121 is effective against a wide range of plant pathogenic fungi: Fusaricum oxysporum, Alternaria alternata, Rhizoctonia solani, Botrvtis cinerea ), Pestalotiopsis microspora and Penicillium italicum. The antibacterial activity against Botrytis cinerea, Microsporum microsporum and Penicillium citrinum were obvious, especially It is the first time to discover the inhibitory effect on the pathogenic fungi of Quasi-Aspergillus. The authors believe that this bacterium has potential applications in biological pesticides and may play an important role in the biological control of plant diseases.