论文部分内容阅读
Difco血琼脂B45培养基,对巴西利什曼种团和利什曼原虫的培养是有用的,可为血清学研究提供大量的巴西利什曼抗原。本文作者以血琼脂为基础,发展了一种液体的B45培养基。将血琼脂基置于-20℃冰冻12小时,而后加无菌蒸馏水稀释成17%浓度, 浸于自来水或置37℃水溶中融化,以无菌操作按1～3ml量分装于无菌的试管内,在-20℃内可保存一年。作者指出,巴西利什曼原虫在这种液体培养基内生长良好,与在常规血琼脂斜面冷凝水内的生长一样,同时在玻璃维亚用此种
Difco Blood Agar B45 medium is useful for the culture of Leishmania brasiliensis and Leishmania serotypes and provides a large number of Brazilian Leishmania antigens for serological research. The authors developed a liquid B45 medium based on blood agar. The blood agar-based frozen at -20 ℃ for 12 hours, then add sterile distilled water diluted to 17% concentration, immersed in tap water or melted at 37 ℃ water soluble, according to aseptic operation by volume of 1 ~ 3ml installed in sterile Test tube, at -20 ℃ can be stored for one year. The authors note that Leishmania brazilian worm grew well in this liquid medium, as did growth in conventional blood agar slant condensation water, while the glassy veil used this