Objective To investigate the effect of endotoxin and tumor necrosis factor (TNF) on hepatic function of rats. Method Prepare hepatic cell suspension by filling male SD rats livers with collagenaseⅠ ,culture in DMEM medium, and add endotoxin and TNF in it. After 24 hours detect GPT, GOT in supatant fluid. Result The activity of GPT and GOT in supatant fluid increased after adding endotoxin and TNF . Conclusion The system of hepatic cell membrane could be damaged severely after acted by endotoxin and TNF.