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目的分析宁波市不同来源(食品、食物中毒、环境物表)金黄色葡萄球菌对常用抗生素的耐药性及肠毒素基因携带情况,为临床合理用药提供依据,预防和控制由该菌引起的相关疾病。方法采用Kirby-Bauer纸片扩散法检测金黄色葡萄球菌对17种临床常用抗生素的敏感性。采用mini-VIDAS检测肠毒素,同时用常规PCR方法扩增分析不同来源金黄色葡萄球菌携带sea、seb、sec、sed、see 5种肠毒素基因情况。结果 89株金黄色葡萄球菌菌株有26株检出肠毒素基因,检出率为29.2%,而来源于食物中毒、食品、环境物表菌株肠毒素基因检出率分别为100.0%、22.9%、10.3%。食物中毒菌株肠毒素基因主要为seb。药敏检测结果显示对青霉素(83.1%)和红霉素(33.7%)耐药性较高,菌株存在多重耐药性。结论食源性金黄色葡萄球菌产毒素能力较强,宁波地区食物中毒菌株肠毒素基因主要为seb。金黄色葡萄球菌对多种药物耐药,提示安全用药的重要性。
Objective To analyze the resistance to common antibiotics and enterotoxin gene of Staphylococcus aureus from different sources in Ningbo City (food, food poisoning and environment object table), provide basis for clinical rational drug use, and prevent and control the correlation caused by the bacteria disease. Methods Kirby-Bauer disk diffusion method was used to detect the susceptibility of Staphylococcus aureus to 17 commonly used antibiotics. The enterotoxin was detected by mini-VIDAS, and the five kinds of enterotoxin genes of serovar, seb, sec, sed and see were also amplified and analyzed by routine PCR. Results Totally 26 strains of 89 strains of Staphylococcus aureus were detected for the gene of enterotoxin, the detection rate was 29.2%. The detection rates of enterotoxin genes in food and environment were 100.0% and 22.9%, respectively, 10.3%. The food poisoning strain enterotoxin gene is mainly seb. The results of susceptibility testing showed that the drug resistance to penicillin (83.1%) and erythromycin (33.7%) was high, and the strain was multi-drug resistant. Conclusion The food-producing Staphylococcus aureus has a strong ability to produce toxins. The enterotoxin gene of food poisoning strains in Ningbo is mainly seb. Staphylococcus aureus is resistant to many drugs, suggesting the importance of safe medication.