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用反相离子对高效液相色谱法对三磷酸腺苷二钠原料（以下简称ＡＴＰ）及４种制剂进行定性定量。色谱条件：流动相为１０％乙腈－水（含１５ｍｍｏｌＩＰＲ－Ａ）；紫外检测波长２５４ｎｍ；色谱柱为国产ＹＷＧ－Ｃ＿（１８）。线性范围０．４～２．０μｇ，相关系数γ＝０．９９９８。测定结果，ＡＴＰ原料、注射液、片剂、胶囊及注射用粉针回收率依次为１００．８％，９９．１１％，１０１．７％，９７．６２％及１０１．０％。本法具有快速灵敏，准确实用等特点。
High performance liquid chromatography with reversed-phase ion pair was used to qualitatively and quantitatively determine the content of adenosine triphosphate (hereinafter referred to as ATP) and four kinds of preparations. Chromatographic conditions: the mobile phase was 10% acetonitrile-water (containing 15mmolIPR-A); UV detection wavelength was 254nm; the chromatographic column was domestic YWG-C_ (18). The linear range of 0.4 ~ 2.0μg, correlation coefficient γ = 0.9998. The results showed that the recoveries of ATP raw materials, injections, tablets, capsules and powder for injection were 100.8%, 99.11%, 101.7%, 97.62% and 101.0%, respectively. This law is fast and sensitive, accurate and practical and so on.