论文部分内容阅读
以黑木相思种子为外植体材料,通过不同的消毒及处理方法,打破种子休眠,在不同培养基上进行试管培养,以期诱导种子在试管内萌芽,利用萌发出来的无菌苗,建立黑木相思的无菌体系,达到增殖扩繁目的。结果表明,种子在0.1%升汞中煮沸5min消毒及打破休眠效果较好,种子在MS+10%椰汁培养基上萌芽效果好,萌芽率达85%以上。将萌芽带茎节部分剪下,转入MS+6BA1.5mg/L+NAA0.05mg/L的培养基中进行丛生芽增殖培养,每个茎节平均长出芽数为3.0;丛生芽生长旺盛,利于进行继代培养和生根成苗。
Taking A. acanthopanax seed as explant material, through different methods of disinfection and treatment, seed dormancy was broken, and tube culture was conducted on different media to induce seeds to germinate in vitro. Using germ-free germ seed to establish black Acacia sterile system to achieve the purpose of proliferation and propagation. The results showed that the seed germinated in 0.1% mercuric chloride for 5 min and the dormancy was better. The seeds germinated well on MS + 10% coconut water medium with germination rate over 85%. The bud part of the stem cut off, transferred MS + 6BA1.5mg / L + NAA0.05mg / L medium for cluster bud proliferation culture, the average number of buds per stem section was 3.0; Conducive to subculture and rooting seedlings.