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目的:探讨人口腔鳞状细胞癌(OSCC)组织中生长抑制因子4(ING4)和缺氧诱导因子-1α(HIF-1α)的表达及其临床意义。方法:选择我院确诊的口腔鳞癌患者共计65例,均经过手术治疗,术前未行放化疗,切除标本经过石蜡包埋切片,同时选取20例正常口腔黏膜作为对照。采用免疫组化S-P法检测人口腔鳞状细胞癌和正常口腔黏膜组织中ING4和HIF-1α的表达,并分析其与OSCC患者临床病理特征的相关性及人口腔鳞状细胞癌组织中ING4和HIF-1α表达的相关性。结果:OSCC组织中ING4的阳性表达率为41.5%(27/65),显著低于正常口腔黏膜组织(P<0.05);OSCC组织中HIF-1α的阳性表达率为64.6%(42/65),显著高于正常口腔黏膜组织(P<0.05)。ING4和HIF-1α的表达与OSCC的病理分级、TNM分期和是否有淋巴结转移显著相关(P<0.05);OSCC组织中,ING4的表达与HIF-1α的表达呈显著负相关(P<0.05)。结论:口腔鳞状细胞癌组织中ING4的表达下调,HIF-1α的表达上调,二者可能通过负向调控作用在口腔鳞状细胞癌的发生、发展、侵袭和转移中发挥着重要作用。
Objective: To investigate the expression of growth inhibitory factor 4 (ING4) and hypoxia inducible factor-1α (HIF-1α) in human oral squamous cell carcinoma (OSCC) and its clinical significance. Methods: A total of 65 patients with oral squamous cell carcinoma diagnosed in our hospital were selected. All of them underwent surgical treatment without radiotherapy and chemotherapy before surgery. Paraffin-embedded sections were excised and 20 normal oral mucosa were selected as control. Immunohistochemical SP method was used to detect the expression of ING4 and HIF-1α in oral squamous cell carcinoma and normal oral mucosa tissues. The correlation between ING4 and HIF-1α in OSCC and clinicopathological characteristics of oral squamous cell carcinoma HIF-1α expression of the correlation. Results: The positive expression rate of ING4 in OSCC tissues was 41.5% (27/65), which was significantly lower than that in normal oral mucosa tissues (P <0.05). The positive expression rate of HIF-1α in OSCC tissues was 64.6% (42/65) , Significantly higher than normal oral mucosa (P <0.05). The expression of ING4 and HIF-1α was significantly correlated with the pathological grade of OSCC, TNM staging and lymph node metastasis (P <0.05). In OSCC, the expression of ING4 was negatively correlated with the expression of HIF-1α (P <0.05) . CONCLUSION: The downregulation of ING4 and the up-regulation of HIF-1alpha in oral squamous cell carcinoma may play an important role in the occurrence, development, invasion and metastasis of oral squamous cell carcinoma through negative regulation.