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目的观察充血性心力衰竭(CHF)患者心肌组织细胞外信号调节激酶(ERK)和磷脂酰肌醇3激酶(PI3K)通路的信号蛋白表达。方法通过手术取材,选择因瓣膜性心脏病接受二尖瓣置换术的CHF患者40例,对照组38例(其中8例为意外伤亡的器官损献者)。免疫沉淀法检测心肌组织ERK、PI3K蛋白表达及磷酸化,α-骨骼肌蛋白表达。结果CHF患者心肌组织呈典型的重构心肌的病理改变,心肌组织ERK蛋白表达光密度值(A)比值(ERK1/β-actin)、(ERK2/β-actin)、PI3K磷酸化比值(p-PI3K/β-actin)、α-骨骼肌蛋白表达A比值(α-skele-tal-actin/β-actin)逐渐增强,且随心功能恶化逐渐增强,CHF组中不同心功能级别者与对照组相比差异均有统计学意义(P<0.05或P<0.01)。结论ERK及PI3K通路共同参与调节CHF患者心肌重构的病理生理过程。
Objective To observe the signal transduction pathway of myocardial extracellular signal-regulated kinase (ERK) and phosphatidylinositol 3-kinase (PI3K) in patients with congestive heart failure (CHF). Methods Forty CHF patients with valvular heart disease undergoing mitral valve replacement surgery and 38 control subjects (8 of whom were organ injury victims) were selected surgically. Immunoprecipitation was used to detect the expression of ERK and PI3K and the phosphorylation and α-skeletal muscle protein expression in myocardial tissue. Results The myocardial tissue of CHF patients showed typical pathological changes of myocardial remodeling. The expressions of ERK1 / β-actin, ERK2 / β-actin, PI3K phosphorylation (p- PI3K / β-actin and α-skeletal-actin / β-actin gradually increased, and with the worsening of cardiac function, the levels of α-skeletal-actin / β- The differences were statistically significant (P <0.05 or P <0.01). Conclusion Both ERK and PI3K pathways are involved in the regulation of myocardial remodeling in patients with CHF.