目的　检测软骨细胞培养液中是否存在抑制血管生长的因子。方法　用酶消化、分离鸡胚关节软骨细胞 ,体外单层培养 ,收集细胞培养液。采用鸡胚绒毛尿囊膜实验检测该培养液对血管的抑制效应。结果　加软骨细胞培养液的实验组血管数为 2 75± 0 99且血管较细、分支少 ;不加样的对照组血管数为 4 5± 1 0 8且血管较粗、分支多。两组有显著差异 (P <0 0 5 )结论　软骨细胞培养液具有抑制血管生长的作用 Objective To determine whether there is a factor that inhibits the growth of blood vessels in the chondrocyte culture medium. Methods Chicken embryo chondrocytes were isolated by enzyme digestion and cultured in vitro. The cell culture medium was collected. The chicken embryo chorioallantoic membrane was used to test the inhibitory effect of the culture fluid on the blood vessels. Results The number of blood vessels in the experimental group plus chondrocyte culture medium was 2 75±0 99 and the blood vessels were fine and with few branches. The number of blood vessels in the control group was 45 ± 108 and the blood vessels were thick and had many branches. There were significant differences between the two groups (P < 0.05) Conclusion Chondrocyte culture fluid has the effect of inhibiting blood vessel growth.