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结合气相色谱仪和乙炔还原(ARA),采用最可能计数法(MPN)和荧光抗体(FA)染色法对盐碱地小麦根际联合固氮菌及优良固氮菌株Pseudomonassp.ChW1和Zoogloeasp.ChW6数量分布进行了测定。结果表明:小麦根际各部位均存在联合固氮菌类群,但数量相对较小、差异较大(102～106个g-1干土或根),分布趋势呈RP>RS>NRS>HP;菌株Pseudomonassp.ChW1是一种相对广谱的联合固氮菌株,在小麦根际不同部位均有分布,数量除在根系表面(RP)较多(105个g-1干根)外,其它部位较少(102～103个g-1干土或根);Zoogloeasp.ChW6菌株是一种分布谱相对较窄的联合固氮菌株,只分布于根表土壤(RS)和根系表面(RP),数量102～104个g-1干土或根。自然状况下,小麦根际固氮菌数量较少,有必要通过使用联合固氮菌接种剂提高小麦根际固氮菌种群数量,增强种群竞争力,增加根际微生物固氮总量。
Combined with gas chromatography and acetylene reduction (ARA), the distribution of Pseudomonas sp. ChW1 and Zoogloeasp. ChW6 in rhizosphere soil of rhizosphere soil and the azotobacter of good nitrogen-fixing bacteria in saline-alkali soil were analyzed by MPN and FA. Determination. The results showed that there was a combination of azotobacter in the rhizosphere of wheat, but the number was relatively small (102 ~ 106 g-1 dry soil or root), and the distribution trend was RP> RS> NRS> HP. Strain Pseudomonas sp.ChW1 is a relatively broad-spectrum combination of nitrogen-fixing strains, which distribute in different parts of wheat rhizosphere. The number of Pseudomonas sp.ChW1 is less than that of the root surface (RP) (105 g-1 dry root) (102-103 g-1 dry soil or roots); Zoogloeasp. ChW6 strain is a relatively narrow distribution of nitrogen-fixing strains, which are only distributed on root surface soil (RS) and root surface (RP) 104 g-1 dry soil or roots. Under natural conditions, the number of nitrogen-fixing bacteria in rhizosphere of wheat is small, it is necessary to increase the number of nitrogen-fixing bacteria in the rhizosphere of rhizosphere by using the combination azotobacter inoculant, enhance the competitiveness of the population and increase the total amount of nitrogen fixation in the rhizosphere.