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目的:探讨喉癌中胰岛素样生长因子结合蛋白-相关蛋白1(IGFBP-rP1)基因启动子区甲基化与蛋白表达之间的关系。方法:采用甲基化特异性PCR方法和免疫组织化学的方法分别检测45例喉癌组织及18例癌旁组织中IGFBP-rP1基因的甲基化状态和蛋白表达情况。结果:喉癌组织IGFBP-rP1启动子区甲基化率为33.3%(15/45),相应癌旁组织甲基化率为5.6%(1/18),喉癌组织中IGFBP-rP1基因甲基化率明显高于癌旁组织(P<0.05)。喉癌组织中IGFBP-rP1蛋白表达显著低于癌旁正常组织(P<0.05),且与其启动子区甲基化状态呈负相关。结论:IGFBP-rP1基因启动子区甲基化导致基因沉默可能是喉癌发生的机制之一。
Objective: To investigate the relationship between methylation and protein expression of insulin-like growth factor-binding protein-related protein 1 (IGFBP-rP1) promoter in laryngeal carcinoma. Methods: Methylation-specific PCR and immunohistochemistry were used to detect the methylation status and protein expression of IGFBP-rP1 in 45 cases of laryngeal carcinoma and 18 cases of paracancerous tissues respectively. Results: The methylation rate of IGFBP-rP1 promoter region in laryngeal carcinoma was 33.3% (15/45) and the corresponding paracancer tissue methylation rate was 5.6% (1/18). The expression of IGFBP-rP1 gene in laryngeal carcinoma Base rate was significantly higher than that in paracancerous tissues (P <0.05). The expression of IGFBP-rP1 protein in laryngeal carcinoma was significantly lower than that in adjacent normal tissues (P <0.05), and negatively correlated with the methylation status of its promoter. Conclusion: Methylation of IGFBP-rP1 gene promoter leading to gene silencing may be one of the mechanisms of laryngeal carcinoma.