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为了初步考察鱼胆草提取物及其活性成分獐牙菜苦苷(含量为61%)的体外抑菌效果和安全性,以及獐牙菜苦苷对α-葡萄糖苷酶活性的影响,试验采用小鼠急性毒性试验的方法测定其安全浓度、试管二倍稀释法测定其体外最小抑菌浓度和紫外分光光度法检测獐牙菜苦苷对α-葡萄糖苷酶活性的抑制率。结果表明:鱼胆草提取物及獐牙菜苦苷以5 g/kg灌胃时,小鼠均无死亡发生;醇沉前后的鱼胆草提取物和獐牙菜苦苷对大肠杆菌的最小抑菌浓度(MIC)均为125 mg/mL,对金黄色葡萄球菌的MIC均为31.25 mg/mL;当獐牙菜苦苷浓度为1 mg/mL时对α-葡萄糖苷酶活性的抑制率为30.19%,浓度为100 mg/mL时抑制率为84.92%。说明可尝试用鱼胆草提取物及獐牙菜苦苷防治由金黄色葡萄球菌等细菌感染引起的疾病,獐牙菜苦苷无毒副作用,有望被研发成一种新的降糖药物。
In order to preliminary study of antibacterial activity and safety of gentian extract and its active ingredient swertiamatidine (content of 61%) in vitro and the effect of swertia glycoside on α-glucosidase activity, Mouse acute toxicity test to determine the safe concentration of test tube double dilution method to determine its minimum inhibitory concentration in vitro and UV spectrophotometry swertia glycoside inhibition of α-glucosidase activity. The results showed that no mortality was found in the mice of the gentian extract and swertia glycoside at 5 g / kg, the minimum of Escherichia coli The inhibitory concentration (MIC) was 125 mg / mL, and the MIC of Staphylococcus aureus was 31.25 mg / mL. The inhibitory rate of α-glucosidase activity when Swertia bitter glycoside concentration was 1 mg / mL Was 30.19%, the inhibition rate was 84.92% at the concentration of 100 mg / mL. Indicating that you can try to use fish gall grass extract and swertia glycosides prevention and control of bacteria caused by Staphylococcus aureus and other diseases caused by swertia glycosides non-toxic side effects, is expected to be developed into a new hypoglycemic drugs.