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本文采用修改Hamburger—salmon体外双层琼脂法与~3H—TdR参入法同时对人类宫颈癌细胞系细胞和卵巢癌细胞进行药物敏测。观察了13例卵巢癌患者用两法测得顺铂体外敏感性与临床疗效的关系。结果表明:HeLa细胞和卵巢癌细胞接种数与克隆产率、CPM值在一定范围内均呈线性相关。用剂量——存活曲线测得阿霉素、顺氯氨铂和长春新碱对HeLa细胞系细胞的IC_(50)值无明显差异。两法测定顺铂对卵巢癌的体外敏感性与临床疗效相关。体内外阳性相关率为88％(8/9);阴性相关率为100％(4/4)。两种方法测得药敏结果无明显差异。我们的实验数据表明~3H～TdR参入法有可能取代双层琼脂法为指导临床化疗选药提供一定依据。
In this article, modified Hamburger-salmon in vitro double-layer agar assay and ~3H-TdR incorporation assay were used to detect drug sensitivity in human cervical cancer cell lines and ovarian cancer cells. The relationship between the sensitivity and clinical efficacy of cisplatin in vitro was measured in 13 patients with ovarian cancer. The results showed that the inoculation number, clone yield, and CPM value of HeLa cells and ovarian cancer cells were linearly correlated within a certain range. There was no significant difference in the IC 50 values of HeLa cell lines between doxorubicin, cisplatin and vincristine as measured by dose-survival curves. The two methods for the determination of cisplatin’s in vitro sensitivity to ovarian cancer are related to clinical efficacy. The positive correlation in vivo and in vitro was 88% (8/9); the negative correlation was 100% (4/4). There was no significant difference in drug sensitivity between the two methods. Our experimental data show that the ~3H~TdR incorporation method may replace the double-layer agar method to provide a basis for guiding clinical chemotherapy and drug selection.