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In order to conduct the fundamental investigation of the effect of 5-FU on HNE1 cells,p33~(ING1) mRNA level in cellular total RNA was detected quantitatively based on MB assay.During the operation,the effects of 5-FU concentrations and treatment time in HNE1 cells and HNE1 cells transfected with p33~(ING1) were measured in vitro.The results were as follows: p33~(ING1) mRNA expression level in tumor cells was enhanced not only by the 5-FU concentration but also by lapse of time.The MTT results also proved that high expression of p33~(ING1) mRNA can increase cell’s sensitivity to chemical drug 5-FU.The detection method based on MB can be used to provide useful evidence quickly and quantitatively for gene expression and new chemical drugs development.
In order to conduct the fundamental investigation of the effect of 5-FU on HNE1 cells, p33 ~ (ING1) mRNA level in cellular total RNA was detected quantitatively based on MB assay. The operation of the effects of 5-FU concentrations and treatment time in HNE1 cells and HNE1 cells transfected with p33 ~ (ING1) were measured in vitro.The results were as follows: p33 ~ (ING1) mRNA expression level in tumor cells was enhanced not only by the 5-FU concentration but also by lapse of time. The MTT results also demonstrated that high expression of p33 ~ (ING1) mRNA can increase cell’s sensitivity to chemical drug 5-FU. The detection method based on MB can be used to provide useful evidence quickly and quantitatively for gene expression and new chemical drugs development.