应用ＳＰＲＩＡ测定抗－ＨＢｓ的方法对乙肝基因疫苗免后３６月的农村小学生进行追踪观察。结果是：美国ｍｅｒｃｋ酵母苗抗－ＨＢｓ阳性率为８０．４９％，美国Ａｍｇｅｎ苗为８３．３３％，国产１－３７血源苗为７７．７８％，国产９２０３－１血源苗为９３．１０％。ＧＭＴ值分别是７４．６２；１４５．７５；１３４．６９；２０１．２９ｍＩｕ／ｍｌ，与免后２４月相比，抗体阳性率略有下降，与免后１２月相比ＧＭＴ值分别下降３８．３８％，４９．９６％，３７．４１％，５６．１９％。证明乙肝基因疫苗三年免疫持久性良好，用乙肝基因疫苗免后三年无需进行大面积加强注时 Using SPRIA to measure anti-HBs, 36-month rural primary school children were followed up for HBV DNA vaccine. As a result, the positive rate of anti-HBs in American merck yeast vaccine was 80.49%, that of American Amgen vaccine was 83.33%, that of domestic 1-37 blood source vaccine was 77.78%, and that of domestic 9203-1 blood vaccine was 93%. 10%. GMT values ​​were 74.62; 145.75; 134.69; 201.29mIu / ml, respectively. The positive rate of antibody decreased slightly compared with that of the first 24 months and the GMT decreased by 38% 38%, 49.96%, 37.41%, 56.19%. Hepatitis B gene vaccine proved three-year immune persistence good hepatitis B gene vaccine-free three years after the need for large-scale intensive injection