论文部分内容阅读
目的:探讨国医大师经验方“抗癌扶正方”的抗肝癌作用及机制。方法:提取抗癌扶正方,针对人肝癌细胞SMMC-7721,经过细胞培养、MTT法、流式细胞仪检该方对肝癌细胞生长抑制率的影响及对突变型P53基因表达的调控作用。结果:抗癌扶正方具有良好的抑制肝癌细胞生长作用,尤其以1mg/mL,10mg/mL剂量组的效果最佳,作用程度与化疗药物类似。此方可以下调凋亡抑制基因突变型P53的表达。结论:抗癌扶正方能够抑制人肝癌细胞SMMC-7721活性。其机理在于降低肝癌凋亡抑制基因的表达,促进细胞凋亡的产生。
Objective: To investigate the anti-hepatocarcinoma mechanism and mechanism of the doctor’s prescription of traditional Chinese medicine “Anticancer Fusheng Recipe”. Methods: The anti-cancer Fuzheng Recipe was extracted from human hepatocellular carcinoma cell line SMMC-7721. The cell growth inhibitory rate and the regulation of the mutant p53 gene expression were determined by MTT assay and flow cytometry. Results: Fangzhengfuzhengfang has a good effect of inhibiting the growth of hepatocellular carcinoma cells, especially in the groups of 1mg / mL and 10mg / mL, the effect is the best, and the degree of action is similar to that of chemotherapy drugs. This side can down-regulate the expression of mutant p53 gene. Conclusion: Fangzheng Fuzheng Fang can inhibit the activity of human hepatoma SMMC-7721 cells. The mechanism is to reduce the expression of hepatocellular apoptosis inhibitory genes and promote apoptosis.