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目的研究干旱胁迫下苦豆子子叶中赖氨酸脱羧酶(lysine decarboxylase,LDC)基因表达量与苦参碱(matrine,MA)和氧化苦参碱(oxymatrine,OMA)含量的关系。方法以不同质量分数PEG 6000胁迫萌动苦豆子种子,72 h后高效液相色谱(HPLC)测定其苦参碱和氧化苦参碱含量,荧光定量PCR(Real-time fluorescence quantitative PCR,q PCR)测定赖氨酸脱羧酶表达量。结果萌动苦豆子种子在轻度胁迫(PEG质量分数<15%)下,子叶中苦参碱和氧化苦参碱含量降低,重度胁迫(PEG质量分数>20%)下,子叶中苦参碱和氧化苦参碱含量升高。荧光定量PCR显示,子叶中赖氨酸脱羧酶表达量随胁迫加剧呈先降后升的趋势,特别是在轻度和重度胁迫下,赖氨酸脱羧酶表达量与苦参碱和氧化苦参碱含量变化一致。结论苦豆子子叶中苦参碱和氧化苦参碱含量与赖氨酸脱羧酶的表达有一定的关系。
Aim To study the relationship between the expression of lysine decarboxylase (LDC) gene and matrine (MA) and oxymatrine (OMA) contents in cotyledon under drought stress. Methods Seed germination of Sophora alopecuroides was induced by different PEG 6000 concentrations. The contents of matrine and oxymatrine were determined by high performance liquid chromatography (HPLC) 72 h later. Real-time fluorescence quantitative PCR (qPCR) Lysine decarboxylase expression level. Results The contents of matrine and oxymatrine in cotyledons were decreased under mild stress (PEG content <15%). Under severe stress (PEG content> 20%), Oxymatrine increased. Fluorescent quantitative PCR showed that the expression of lysine decarboxylase in cotyledons tended to decrease first and then increase with the increase of stress, especially under mild and severe stress, the expression of lysine decarboxylase was significantly correlated with matrine and oxymatrine Alkali content changes consistent. Conclusion The contents of matrine and oxymatrine in the cotyledon of Sophora alopecuroides are related to the expression of lysine decarboxylase.