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目的 在超微结构水平观察两种神经递质在纤维终末内的共存或一种神经递质与其相应受体之间的关系。 方法 包埋前免疫电镜双重标记技术———酶标法和免疫金 银标记法相结合的方法。 结果 在免疫反应双重标记的纹状体切片上 ,电镜下观察到大量的SP样 (过氧化物酶免疫反应产物 )阳性终末和SP受体 (SPR ,免疫金 银标记颗粒 )样阳性神经元的胞体和树突 ,同时可见部分SP样阳性轴突终末分别与SPR样阳性神经元的胞体或树突形成对称性轴 体或轴 树突触联系。而在三叉神经脊束核尾侧亚核切片上 ,电镜下可观察到大量的两种囊泡膜谷氨酸转运体 ,即DNPI样 (过氧化物酶免疫反应产物 )和VGluT1样 (免疫金 银标记颗粒 )阳性轴突终末 ,同时还观察到DNPI样和VGluT1样双标的轴突终末与阴性树突形成非对称性突触。 结论 包埋前免疫电镜双重标记技术敏感性较高 ,组织的抗原性保存好 ,特别是在神经解剖学研究中 ,用于研究两种神经递质在同一个细胞或终末内的共存或分析神经递质与其相应受体之间的联系中有独到之处。
Objective To observe the coexistence of two neurotransmitters in the fiber terminal or the relationship between one neurotransmitter and its corresponding receptor at the ultrastructure level. Methods before embedding immune electron microscopy double labeling technique --- ELISA method and immunogold labeling method combined. Results A large number of SP-like (peroxidase-immunoreactive product) -positive terminal and SP receptor (SPR) -positive neurons were observed on the double labeled striatum of immunoreactive cells Of the somatic cells and dendrites. At the same time, some SP-like axon terminals formed symmetrical axial or axonal synaptic contacts with the soma or dendrites of SPR-like neurons respectively. A large number of vesicular glutamate transporters, DNPI-like (peroxidase immunoreactive products) and VGluT1-like (immunogold Silver labeled particles) -positive axon terminals, as well as DNPI-like and VGluT1-like double-labeled axon terminals forming negative synapses with the negative dendrites. Conclusions Immunoelectron microscopy double labeling before embedding is more sensitive and histocompatibility preserved, especially in the neuroanatomy study for the co-existence or analysis of two neurotransmitters in the same cell or terminal Neurotransmitters and their corresponding receptors in the relationship between the unique.