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选取初始体质量为(35.53±0.62)g的团头鲂180尾,随机分为3个处理组,每组3个重复,每重复20尾。3个处理组即为1个对照组(CON组)和2个试验组(BS-1组与BS-2组),分别投喂不添加枯草芽孢杆菌的基础饲料(CON组),添加500 g/T枯草芽孢杆菌的试验料(BS-1组)和添加1 000 g/T枯草芽孢杆菌的试验料(BS-2组)。为期8周的养殖试验结束后,采样测定生长性能指标和抗氧化指标。结果表明:与CON组相比,BS-1组和BS-2组团头鲂的增重率和特定生长率显著提高(P<0.05),饲料系数显著降低(P<0.05),存活率增加,但差异不显著(P>0.05);BS-1组和BS-2组团头鲂的血清和肝超氧化歧化酶和谷胱甘肽过氧化物酶活性均显著高于CON组(P<0.05),且肝中丙二醛含量显著低于CON组(P<0.05)。由此可见,饲料中添加枯草芽孢杆菌能提高团头鲂的生长性能和抗氧化功能,增强机体的防御能力,进而促进机体健康。
180 breams with initial body mass of (35.53 ± 0.62) g were randomly divided into three treatment groups with 3 replicates in each group with 20 mice in each replicate. The three treatment groups were one control group (CON group) and two experimental groups (BS-1 group and BS-2 group), fed with basal diet without adding Bacillus subtilis (CON group) and 500 g / T Bacillus subtilis test material (BS-1 group) and added 1000 g / T Bacillus subtilis test material (BS-2 group). After a period of 8 weeks of aquaculture testing, samples were taken to measure the growth performance and the antioxidant index. The results showed that compared with the CON group, the weight gain rate and specific growth rate of BS-1 and BS-2 groups were significantly increased (P <0.05), feed coefficient was significantly lower (P <0.05), the survival rate was increased, But the difference was not significant (P> 0.05). The superoxide dismutase and glutathione peroxidase activities in serum and liver of group BS-1 and group BS-2 were significantly higher than that of group CON (P <0.05) , And liver malondialdehyde content was significantly lower than CON group (P <0.05). Thus, the addition of Bacillus subtilis to feed can improve the growth performance and antioxidant function of the bream, enhance the body’s defensive ability, thereby promoting the body’s health.