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玉米组织蛋白质的提取及其浓度测定是进行玉米蛋白质组学研究的重要环节。本文对四川地区常见9个玉米品种籽粒蛋白质浓度进行了分析,利用改进Tris法提取籽粒的总蛋白,使用NanoDrop 2000c超微量分光光度计测定蛋白质浓度。以期为筛选出适合四川地区种植的优质蛋白玉米品种和玉米蛋白质组学研究提供参考。
Corn protein extraction and determination of concentration of corn proteomics is an important part of the study. In this paper, the grain protein concentrations of nine maize cultivars in Sichuan Province were analyzed. The total protein of the grains was extracted by modified Tris method and the protein concentration was determined by NanoDrop 2000c ultramicro spectrophotometer. In order to provide a reference for the screening of high-quality protein maize varieties and corn proteomics suitable for planting in Sichuan.