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A-蛋白和羊抗兔血清应用于免疫电镜,检测马铃薯X病毒(PVX)粗汁液,灵敏度比诱捕修饰法高;而且加羊抗兔血清后,病毒粒体比诱捕修饰法明显加粗,在电镜放大2500倍时,就能清晰地观察到。用诱捕双修饰法检测PVX,证实在寄主植物的叶、茎和根中均存在,以叶内含量最高。接种在普通烟上第三天,接种叶就能检测到PVX,接种15天后,寄主体内病毒能达到较高浓度,而且高浓度一直可保持2个月以上;PVX在不同寄主中的含量不同,以普通烟中病毒浓度最高,心叶烟、番茄和黄花烟中次之;昆诺阿藜、千日红和大椒中含毒量极抵。用诱捕双修饰法检测马铃薯薯块休眠芽中的病毒获得成功。
A-protein and goat anti-rabbit serum were applied to immunoelectron microscopy to detect the crude juice of potato virus X (PVX), and the sensitivity was higher than that of trapping modification method. Moreover, after the addition of sheep anti-rabbit serum, Electron microscope 2500 times magnification, you can clearly observe. PVX was detected by trapping double-modification method and confirmed to be present in the leaves, stems and roots of the host plants with the highest content in the leaves. On the third day after inoculation on ordinary tobacco, PVX could be detected in inoculated leaves. After 15 days of inoculation, the virus in the host could reach a higher concentration, and the high concentration of PVX could be maintained for more than two months. The contents of PVX in different hosts were different, The highest concentration of ordinary tobacco virus, heart leaf tobacco, tomato and yellow smoke second; quinoa, thousands of red and pepper in the amount of extremely arrived. Detection of virus in dormant bud of potato tuber by double trapping method.