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目的探讨Jagged1在体外培养的海马放射状胶质细胞增殖和向神经元分化中的作用。方法体外培养海马放射状胶质细胞,在培养液中加入Notch信号通路的激动剂Jagged1和(或)抑制剂{氮-[氮-(3,5-二氟苯乙酰)-L-丙氨酰]-S-苯基甘氨酸丁酯,DAPT},将细胞分为空白对照组、Jagged1组、Jagged1联合DAPT组、DAPT组;细胞计数试剂盒8(CCK-8)检测各组细胞活力;免疫荧光法检测脑脂结合蛋白(BLBP)/Ki67双标阳性细胞数及分化所得的微管相关蛋白-2(MAP-2)阳性细胞数。结果 Jagged1组中细胞活力明显高于其他各组,并且Jagged1组中BLBP/Ki67双标阳性细胞及分化所得的MAP-2阳性细胞数也多于其他各组。结论 Jagged1能够促进体外培养的海马放射状胶质细胞增殖,并且能够促进其更多地向神经元分化。
Objective To investigate the role of Jagged1 in proliferation and neuronal differentiation of hippocampal astrocytes cultured in vitro. Methods The hippocampal radial glial cells were cultured in vitro. Jagged1 and / or inhibitor of Notch signaling pathway {nitrogen- [N- (3,5-difluorophenylacetyl) -L-alanyl] S-phenylglycine butyl ester, DAPT}, the cells were divided into blank control group, Jagged1 group, Jagged1 combined with DAPT group, DAPT group; cell counting kit 8 (CCK-8) The number of BLBP / Ki67 double positive cells and the numbers of microtubule associated protein-2 (MAP-2) positive cells were detected. Results The cell viability in Jagged1 group was significantly higher than that in other groups, and the number of BLBP / Ki67 double positive cells and the number of MAP-2 positive cells differentiated in Jagged1 group were also more than those in other groups. Conclusion Jagged1 can promote hippocampal astrocyte proliferation in vitro and promote its differentiation into neurons.