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目的:探讨MACC1和c-MET与肾癌的相关性,明确两者之间调控关系及对肾癌Caki-1细胞增殖的影响。方法:应用荧光定量PCR检测67例肾癌及相应癌旁正常组织中MACC1和c-MET mRNA的表达。设计并合成MACC1基因特异性的siRNA,转染肾癌Caki-1细胞,检测转染后MACC1和c-MET蛋白的表达以及Ca-ki-1细胞的细胞抑制率。结果:肾癌组织中的MACC1和c-MET mRNA表达较癌旁正常组织中均有明显增加(P<0.05),且两者呈正相关。siRNA-MACC1能够明显抑制Caki-1细胞中MACC1蛋白的表达,P<0.05。MACC1蛋白表达抑制的Caki-1细胞中c-MET蛋白的表达亦降低(P<0.05),细胞抑制率明显升高,P<0.05。结论:MACC1和c-MET都与肾癌相关,MACC1能够调节下游基因c-MET的表达,影响肾癌细胞的增殖能力,发挥促进肿瘤生长的作用。
Objective: To investigate the correlation between MACC1 and c-MET and renal cell carcinoma and to clarify the relationship between them and their effect on the proliferation of Caki-1 cells. Methods: The mRNA expressions of MACC1 and c-MET in 67 cases of renal cell carcinoma and adjacent normal tissues were detected by real-time fluorescence quantitative PCR. The siRNA targeting MACC1 gene was designed and synthesized. The expression of MACC1 and c-MET protein and the cell inhibition rate of Ca-ki-1 cells were detected after transfected with Caki-1 cells. Results: The expressions of MACC1 and c-MET mRNA in renal cell carcinoma tissues were significantly higher than those in adjacent normal tissues (P <0.05), and both were positively correlated. siRNA-MACC1 significantly inhibited MACC1 protein expression in Caki-1 cells, P <0.05. The expression of c-MET protein in Caki-1 cells was also decreased (P <0.05), and the cell inhibition rate was significantly increased, P <0.05. Conclusion: Both MACC1 and c-MET are associated with renal cell carcinoma. MACC1 can regulate the expression of downstream gene c-MET, affect the proliferation of renal cell carcinoma and promote tumor growth.