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目的 观察细胞稳定钙离子内流抑制剂羧基胺基咪唑（ＣＡＩ） 抑制内皮细胞构建新生血管和人肝癌诱导血管形成的作用。方法 应用ＭＴＴ、Ｂｏｙｄｅｎ 培养皿和人工基质，研究ＣＡＩ对人脐静脉内皮细胞（ＨＵＶＥＣ）增殖、迁移、降解基质和构建血管能力的影响，利用ＬＣＩＤ２０ 瘤株裸鼠角膜模型观察ＣＡＩ对人肝癌诱导血管形成能力的影响。结果 ＣＡＩ的浓度超过１０ ｍｍｏｌ／Ｌ 时，内皮细胞的增殖明显抑制；与对照组相比，ＣＡＩ组细胞的迁移、降解基质和构建血管的能力下降（ Ｐ＜ ０．０５）；２ μｌ ＣＡＩ（１００μｍｏｌ／Ｌ）软膏动物涂眼，每日２ 次，ＣＡＩ组动物角膜新生血管的数目和长度分别小于和短于对照组（ Ｐ＜０．０５）。结论 ＣＡＩ能够明显抑制内皮细胞构建新生血管和人肝癌诱导的血管形成。
Objective To observe the effects of stable calcium ion influx inhibitor carboxylamine imidazole (CAI) on endothelial cells in the formation of neovascularization and human hepatoma-induced angiogenesis. METHODS: The effects of CAI on the proliferation, migration, degradation of stroma and vascularization of human umbilical vein endothelial cells (HUVEC) were studied using MTT, Boyden dishes and artificial matrix. The observation of CAI on human hepatocellular carcinoma was performed using a nude mouse corneal model of LCID-20 tumor strain. Induced angiogenesis effects. Results When the concentration of CAI exceeded 10 mmol/L, the proliferation of endothelial cells was significantly inhibited. Compared with the control group, the CAI group’s ability to migrate, degrade the matrix, and construct blood vessels decreased (P < 0.05); 2 μl CAI ( The number and length of corneal neovascularization in the CAI group were smaller and shorter than those in the control group (P<0.05). Conclusion CAI can significantly inhibit the formation of neovascularization by endothelial cells and human hepatoma-induced angiogenesis.