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目的评价和比较不同肠球菌种水平鉴定方法的准确性,了解北京市集贸市场生鲜猪肉肠球菌的种水平分布及耐药特征。方法利用基质辅助激光解吸电离飞行时间质谱(MALDI-TOF-MS)和VITEK 2 COMPACT两种方法对北京市集贸市场生鲜猪肉肠球菌开展种水平鉴定,两种方法鉴定结果不一致的菌株使用API 20 Strep、16S rRNA和23S rRNA序列分析再次鉴定。比较不同种肠球菌对10种临床常用抗生素的药物敏感性。结果在5种肠球菌种水平鉴定方法中,16S rRNA序列分析和最终结果的符合率最高,为100.0%;VITEK 2 COMPACT符合率最低,仅为4.0%(1/25)。在86株肠球菌中,粪肠球菌占82.6%(71/86),其次为希拉肠球菌占14.0%(12/86)。粪肠球菌对环丙沙星(CIP)的耐药率高于希拉肠球菌,差异有统计学意义(χ~2=10.751,P<0.01);对四环素(TET)、高浓度链霉素(HLSR)和高浓度庆大霉素(HLGR)的耐药率均高于希拉肠球菌,但差异无统计学意义(TET:χ~2=3.865,P>0.05;HLSR:χ~2=1.608,P>0.05;HLGR:χ~2=0.553,P>0.05),但对红霉素(ERY)和氯霉素(CHL)的耐药率明显低于希拉肠球菌,差异有统计学意义(ERY:χ~2=20.244,P<0.01;CHL:χ~2=14.139,P<0.01)。结论 16S rRNA和MALDI-TOF-MS序列分析技术在肠球菌种水平快速鉴定上有较高准确性,不同种属肠球菌呈现不同耐药特征,本研究为有效监测食源性耐药肠球菌的传播和流行提供了科学数据。
Objective To evaluate and compare the accuracy of different Enterococcus species level identification methods and understand the species distribution and drug resistance characteristics of fresh Enterococcus Enteritidis in Beijing market. Methods Two kinds of methods of matrix-assisted laser desorption / ionization time of flight mass spectrometry (MALDI-TOF-MS) and VITEK 2 COMPACT were used to identify the species of fresh Enterococcus enteritidis in Beijing market. The results of the two methods were inconsistent. 20 Strep, 16S rRNA and 23S rRNA sequence analysis again identified. Compare different kinds of enterococci to 10 kinds of commonly used antibiotics, drug sensitivity. Results The highest coincidence rate of the 16S rRNA sequence analysis and the final result was 100.0% among the five Enterococcus species. The lowest coincidence rate of VITEK 2 COMPACT was only 4.0% (1/25). Of the 86 enterococci, Enterococcus faecalis accounted for 82.6% (71/86), followed by Enterococcus hyracula 14.0% (12/86). Enterococcus faecalis ciprofloxacin (CIP) resistance rate was higher than that of Enterococcus sativa, the difference was statistically significant (χ ~ 2 = 10.751, P <0.01); tetracycline (TET), high concentrations of streptomycin HLSR) and high concentration of gentamicin (HLGR) were higher than those of Enterococcus saicifaciens, but the difference was not statistically significant (TET: χ ~ 2 = 3.865, P> 0.05; (P> 0.05; HLGR: χ ~ 2 = 0.553, P> 0.05). However, the resistance rates to erythromycin and chloramphenicol were significantly lower than those from H. pylori, the difference was statistically significant (ERY : χ ~ 2 = 20.244, P <0.01; CHL: χ ~ 2 = 14.139, P <0.01). Conclusion 16S rRNA and MALDI-TOF-MS sequence analysis techniques have high accuracy in the rapid identification of enterococcal species, and different species of enterococci present different drug resistance characteristics. In this study, to effectively monitor enterococci enterococci Communication and popularity provide scientific data.