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目的:探讨辛夷对酒精性肝损伤小鼠的保护作用及作用机制。方法:用连续灌服白酒的方法建立小鼠酒精性肝损伤模型。将60只小鼠随机分为6组:空白对照组、酒精模型组、联苯双酯组(150 mg/kg)和辛夷制剂组(2.0、4.0、8.0g/kg),连续灌胃4周。肝组织HE染色观察病理改变,测定肝指数,血清丙氨酸氨基转移酶(ALT)和天冬氨酸氨基转移酶(AST)活性及肝匀浆超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量,RT-PCR检测肝组织CD14、TNF-αmRNA表达。结果:与模型组相比,辛夷组(4.0、8.0g/kg)小鼠肝细胞形态基本正常;ALT、AST活性、MDA含量、CD14、TNF-αmRNA表达水平显著降低,SOD活性显著增高。结论:辛夷对小鼠酒精性肝损伤具有明显的保护作用,可能与降低肝细胞氧化应激和CD14、TNF-αmRNA表达有关。
Objective: To investigate the protective effect of Magnolia bark on alcoholic liver injury in mice and its mechanism. Methods: A model of alcoholic liver injury in mice was established by continuous irrigation of liquor. 60 mice were randomly divided into 6 groups: control group, alcohol model group, bifendate group (150 mg / kg) and Xin Yi preparation group (2.0,4.0,8.0 g / kg) . Liver histopathological changes were observed by HE staining. Liver index, serum ALT and AST activities, SOD activity in liver homogenate and activity of malondialdehyde Aldehyde (MDA) content, and the expression of CD14 and TNF-αmRNA in liver tissue were detected by RT-PCR. Results: Compared with the model group, the morphological changes of hepatocytes in mice treated with Magnolia bungeanum (4.0, 8.0 g / kg) were basically normal. The activities of ALT, AST, MDA, CD14 and TNF-α mRNA were significantly decreased and SOD activity was significantly increased. CONCLUSION: Xinyi has a significant protective effect on alcoholic liver injury in mice, which may be related to the reduction of oxidative stress and expression of CD14 and TNF-αmRNA in hepatocytes.