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目的：为获得能在体外稳定繁殖的细粒棘球蚴细胞系。方法：用研磨法将绵羊源细粒棘球蚴生发层处理得分散的生发细胞，将其在含１０％—２０％小牛血清的ＲＰＭＩ１６４０培养基在２４孔培养板和包被胶原的２４孔细胞培养板上交替培养至１４代，随后在常规２４孔培养板上连续传代培养；用倒置显微镜观察细胞形态及生长繁殖情况；将培养细胞接种ＢＡＬＢ／ｃ小鼠以观察其形成继发性包囊的能力；用ＥＬＩＳＡ法研究细胞系抗原的免疫学特征。结果：生发层细胞已在体外连续培养７５代以上，生长良好；细胞系细胞呈圆形，具有形成合胞体乃至类组织块的趋势，在４℃冰箱中至少可保存半个月，在液氮中至少可保存１０个月；将细胞接种ＢＡＬＢ／ｃ小鼠后３个月至７个月，解剖未见细粒棘球蚴包囊生长；细胞系抗原可和抗生发层体抗原、原头节可溶性抗原、囊液抗原的小鼠血清及人工感染原头节的阳性小鼠血清起反应。结论：细粒棘球蚴生发层细胞系已经建立。
OBJECTIVE: To obtain Echinococcus granulosus cell line that can be stably propagated in vitro. Methods: The germinal cells of Echinococcus granulosus were treated by grinding method. The cells were cultured in RPMI 1640 medium containing 10% -20% fetal bovine serum in 24-well culture plate and collagen-coated 24-well The cells were cultured on the plate for 14 passages alternately and subsequently subcultured on a conventional 24-well plate. The cell morphology and the growth and reproduction were observed under an inverted microscope. The cultured cells were seeded into BALB / c mice to observe the formation of secondary cells Cystic ability; using ELISA method to study the immunological characteristics of cell line antigen. Results: The germinal layer cells had been continuously cultured for more than 75 passages in vitro and grew well. The cell lines were round with the trend of forming syncytia and even tissue-like blocks. They could be stored in refrigerator at 4 ℃ for at least half a month, At least 10 months can be preserved; the cells were inoculated BALB / c mice after 3 months to 7 months, dissection did not see Echinococcus granulosus cyst growth; cell line antigen and anti-germinal layer antigen, the original head Section of soluble antigen, cystic fluid antigen of mouse serum and artificial infection of the original head of the positive mouse serum reaction. Conclusion: Echinococcus granulosus germinal layer cell line has been established.